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Metallomics : Analytical Techniques and Speciation Methods
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Find it at other libraries via WorldCat Limited preview. According to Padilha, the study managed to optimize metal speciation methods by making use of a rather recent field known as metallomics. A combination of proteomics and metal detection techniques, metallomics aims to determine the distribution of metal and metalloid species and to clarify the physiological and functional characteristics of biomolecules whose structures contain metal ions.
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Padilha said that there are two ways for a protein to carry metals. The first is when the metal is part of the protein molecule itself — such as hemoglobin, a metalloprotein that has iron atoms that transport oxygen. The other method of transport is when the metal or metalloid binds to the protein through non-specific ligands, forming what is known as a metal-binding protein.
It is from this last group that the research team chose proteins capable of acting as possible biomarkers for the presence of mercury in the fish. Isoforms are different forms of the same protein, but they are encoded by different genes and present small differences in their peptide sequences.
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The definition of an effective biomarker will be made in a future stage of the project. In this part of the project, the researchers improved the methods for metalloproteomics study of mercury, according to the Unesp professor. There, researchers at the Botucatu laboratories handled one of the most complicated parts of the work: extracting the proteins.
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Permissions Icon Permissions. Abstract Elemental metabolomics is quantification and characterization of total concentration of chemical elements in biological samples and monitoring of their changes. Issue Section:. You do not currently have access to this article.
Online Metallomics Analytical Techniques And Speciation Methods
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